Select publications
Altas B, Romanowski AJ, Bunce G, Poulopoulos A. Neuronal mTOR Outposts: Implications for Translation, Signaling, and Plasticity. (2022) Front. Cell. Neurosci. doi: 10.3389/fncel.2022.853634.
Richardson RR, Steyert M, Inen J, Khim S, Romanowski AJ, Altas B, Poulopoulos A. Cas9 fusions for precision in vivo editing. (2020) bioRxiv, doi: 10.1101/2020.07.15.199620
Poulopoulos A*†, Murphy AJ*, Ozkan A, Davis P, Hatch J, Kirchner R, Macklis JD†. Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex. (2019) Nature, 565(7739):356-360.
Poulopoulos A (editor). Synapse Development. (2017) Methods Mol Biol, 1538.
Poulopoulos A. ‘Holistic’ synaptogenesis. (2010) Biochem Soc Trans, 38(2):511-5.
Poulopoulos A*, Aramuni G*, Meyer G, Soykan T, et al. Neuroligin 2 drives postsynaptic assembly at perisomatic inhibitory synapses through gephyrin and collybistin. (2009) Neuron, 63(5):628-42.
Complete publication list on Google Scholar
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Never heard of “mTOR outposts”? Now you have!
Read all about our postulate of the curious little things called “mTOR outposts” in this Hypothesis & Theory paper just out in Frontiers in Cellular Neuroscience! Neuronal mTOR Outposts: Implications for Translation, Signaling, and Plasticity Happy day for Bek, Andrea, Garrett, and Alex. Appreciations to Helen Bateup and Akira Yoshii for very constructive reviewing; to …
Continue reading “Never heard of “mTOR outposts”? Now you have!”
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How an mTOR pathway gene causes epilepsy in a pedigree dating from 1727
Congratulations to Philip Iffland and the Peter Crino Lab –with help from PouLab grad student Andrea Romanowski among the collaborator team– for the publication of a massive piece of work just out in Brain, spanning the fields of human genetics, cell biology, genome editing, electrophysiology, and brain development to identify the gene (NPRL3) and mechanisms …
Continue reading “How an mTOR pathway gene causes epilepsy in a pedigree dating from 1727”
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Growth cone sorting in Nature Protocols
Our protocol on subtype-specific growth cone sorting by Engmann and Hatch et al. is out on Nature Protocols today from Alex’s old team in the Macklis lab@Harvard! Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.
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Ryan’s article on advancing scientific excellence through inclusivity at the NIH BRAIN Initiative is out in Neuron!
Lab alum, Ryan R. Richardson, now a AAAS STP fellow at the NIH BRAIN Initiative, together with colleagues deputy Director Andrea C. Beckel-Mitchener, Director John Ngai, and program Director Devon C. Crawford, published a paper today in Neuron, outlining how BRAIN’s mission for scientific excellence is empowered by tapping into the full spectrum of diverse …
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Congrats to Ryan and co. for “Cas9 fusions for precision in vivo editing” out on the bioRxiv!
The lab’s first pub is a nifty piece of synth bio for genome editing the brain. Richardson et al. describe a platform to test and develop new high-precision genome editing reagents. Some of our new CRISPR fusions, like eRad18-Cas9-CtIP with linear donors, showed up to 45-times higher accuracy at point-mutation editing compared to vanilla CRISPR. Another …
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Our paper on subcellular -omics from cortical projections out today in Nature
Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex … Hurray!
