Select publications
Richardson RR, Steyert M, Khim SN, Crutcher GW, Brandenburg C, Robertson C, Romanowski AJ, Inen J, Altas B, Poulopoulos A. Enhancing precision and efficiency of Cas9-mediated knockin through combinatorial fusions of DNA repair proteins. (2023) CRISPR J, 6(5):447-461
Ament SA, Poulopoulos A. “The Brain’s Dark Transcriptome: Sequencing RNA in distal compartments of neurons and glia“. (2023) Curr Opin Neurobiol, 81:102725
Altas B, Tuffy LP, Patrizi A, Dimova K, Soykan T, Romanowski AJ, Robertson CD, Khim SN, Bunce GW, Ambrozkiewicz MC, Oleksandr Yagensky, Dilja Krueger-Burg, Matthieu Hammer, Hsiao HH, Laskowski PR, Dyck L, Sassoè-Pognetto M, Chua JJE, Urlaub H, Jahn O, Brose N, Poulopoulos A. Phosphorylation Determines Whether Neuroligin-3 is at Excitatory or Inhibitory Synapses in Different Regions of the Brain. (2022) bioRxiv, doi: 10.1101/2022.07.23.501257
Altas B, Romanowski AJ, Bunce G, Poulopoulos A. Neuronal mTOR Outposts: Implications for Translation, Signaling, and Plasticity. (2022) Front. Cell. Neurosci. doi: 10.3389/fncel.2022.853634
Poulopoulos A*†, Murphy AJ*, Ozkan A, Davis P, Hatch J, Kirchner R, Macklis JD†. Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex. (2019) Nature, 565(7739):356-360
Poulopoulos A (editor). Synapse Development. (2017) Methods Mol Biol, 1538.
Complete publication list on Google Scholar
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Cas9-RC on the cover of The CRISPR Journal!
Ryan Richardson’s paper describing the development of Cas9-RC, a new CRISPR agent with increased performance for knockin, is out in the October 2023 issue of The CRISPR Journal. Not only that, but the paper got the cover! Kudos to Cheryl Brandenburg for the beautiful image of knockin neurons and astrocytes using Cas9-RC in the developing…
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The Brain’s Dark Transcriptome
Did you know that an estimated 40% of RNA in the brain is not captured by single-cell RNAseq? Explore the intriguing world of the Brain’s Dark Transcriptome in our latest review, published in Current Opinion in Neurobiology! Check out the other great reviews in this special issue on Developmental Neuroscience edited by Debby Silver and Franck Polleux!
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Phosphorylation of Neuroligin-3
Our work on how the synaptic adhesion molecule Neuroligin-3 is targeted to either excitatory or inhibitory synapses based on phosphorylation is now available on the bioRxiv! Congrats to Bekir Altas, Liam Tuffy, Annarita Patrizi and the rest of the team in this international collaboration between the University of Maryland School of Medicine, the Max Planck…
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Never heard of “mTOR outposts”? Now you have!
Read all about our postulate of the curious little things called “mTOR outposts” in this Hypothesis & Theory paper just out in Frontiers in Cellular Neuroscience! Neuronal mTOR Outposts: Implications for Translation, Signaling, and Plasticity Happy day for Bek, Andrea, Garrett, and Alex. Appreciations to Helen Bateup and Akira Yoshii for very constructive reviewing; to…
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How an mTOR pathway gene causes epilepsy in a pedigree dating from 1727
Congratulations to Philip Iffland and the Peter Crino Lab –with help from PouLab grad student Andrea Romanowski among the collaborator team– for the publication of a massive piece of work just out in Brain, spanning the fields of human genetics, cell biology, genome editing, electrophysiology, and brain development to identify the gene (NPRL3) and mechanisms…
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Growth cone sorting in Nature Protocols
Our protocol on subtype-specific growth cone sorting by Engmann and Hatch et al. is out on Nature Protocols today from Alex’s old team in the Macklis lab@Harvard! Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.
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Ryan’s article on advancing scientific excellence through inclusivity at the NIH BRAIN Initiative is out in Neuron!
Lab alum, Ryan R. Richardson, now a AAAS STP fellow at the NIH BRAIN Initiative, together with colleagues deputy Director Andrea C. Beckel-Mitchener, Director John Ngai, and program Director Devon C. Crawford, published a paper today in Neuron, outlining how BRAIN’s mission for scientific excellence is empowered by tapping into the full spectrum of diverse…
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Congrats to Ryan and co. for “Cas9 fusions for precision in vivo editing” out on the bioRxiv!
The lab’s first pub is a nifty piece of synth bio for genome editing the brain. Richardson et al. describe a platform to test and develop new high-precision genome editing reagents. Some of our new CRISPR fusions, like eRad18-Cas9-CtIP with linear donors, showed up to 45-times higher accuracy at point-mutation editing compared to vanilla CRISPR. Another…
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Our paper on subcellular -omics from cortical projections out today in Nature
Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex … Hurray!
