The lab’s first pub is a nifty piece of synth bio for genome editing the brain. Richardson et al. describe a platform to test and develop new high-precision genome editing reagents. Some of our new CRISPR fusions, like eRad18-Cas9-CtIP with linear donors, showed up to 45-times higher accuracy at point-mutation editing compared to vanilla CRISPR. Another step toward direct in vivo knockin and in situ gene therapy approaches!
We’ve all had extra time to think about stuff we might otherwise not. Garrett’s neighborhood seems to be thinking a lot about brains! Now his neighbors get a shot at some answers…!
And now some good news for a change… BIG CONGRATS to Marilyn Steyert and Uriel Jean-Baptist for cleaning house on their graduate school applications!
Come next semester, Marilyn will be a PhD student in the Biomedical Sciences (BMS) Program at UC San Francisco, and Uriel will be a PhD student in the Biological & Biomedical Sciences Program (BBSP) at UNC Chapel Hill. We will miss you both, and you both will make us proud!
(photo by T. Soykan)
The lab has fun recreating Raphael’s “The School of Athens” in Chicago, while presenting at the 2019 meeting of the Society for Neuroscience!
Ryan, Marilyn, and Jeffrey unveiled their new approach to high efficiency in vivo genome engineering with in situ CRISPR:
Bek, Andrea, Noury, and Uriel debuted their findings on how a mutation in a family with intellectual disability affects the dynactin complex and cortical circuit wiring:
Alex presented new findings on how phosphorylation of Neuroligin adhesion molecules determines synapse specificities:
And how reciprocal adhesion gradient matching guides the development of cortical circuitry:
… and Garrett stashed us some sweet top-shelf probes…
Garrett Bunce from the Program in Neuroscience joins the lab. Garrett will apply his training in Systems and Computational Neuroscience to develop recording strategies for listening in on in situ genome engineered rat brains… Welcome Garrett!
Our lab received an NIH Director’s New Innovator Award, hurray!!!
The NIH Common Fund just announced the recipients of its 2019 High-Risk, High-Reward Research Program. The award comes with generous funding that will allow our research to keep being strange. Thanks to all!
image from nih.gov
We are very excited to welcome the newest PouLab members, three talented postbac scholars from UMB’s prestigious STAR-PREP program will become the lab’s youngest investigators! Jefferey Inen, mouse geneticist & cancer biologist extraordinaire from UMBC, will work with Ryan Richardson; Saovleak “Noury” Khim, all-round development-to-regenerative neuroscientist from Temple, and Uriel Jean-Baptiste, hardcore structure-function biochemist from Florida State, will work with Bek Altas. Welcome to your new lab, enjoy your research, and get lots of great data!
Congratulations to Andrea Romanowski for passing her qualifying exam and advancing to PhD candidacy with flying colors!
Ryan Richardson presented his work on “Optimizing HDR efficiency and biallelic modification for in vivo knock-in” at the Keystone Symposium on Genome Engineering at Victoria, BC, Canada.
Andrea and Garrett spreading the love for neuroscience at Windsor Hills Elementary and Middle School!
Learn more at UMB NOVA (Neuroscience Outreach and Volunteer Association)