Introducing PegAssist, a new web tool for Prime editing!

For those itching to try the latest CRISPR genome editing technology, Marilyn Steyert, Ryan Richardson, Corey Flynn and co. have put together PegAssist.app an open web tool (and soon-to-be-available plasmid set) to design and construct Prime editing reagents.

Prime editing, developed by the Liu Lab (Anzalone et al. Nature 2019), enables “search-and-replace genome editing” using a Cas9 and Reverse Transcriptase fusion and hybrid pegRNA. The PegAssist web app guides you through pegRNA design for your favorite genome mutation. Help us beta test PegAssist and let us know how to make it better!

Corinne Martin joins the team as the lab’s first Nathan Schnaper Intern!

Corinne Martin, a rising sophomore majoring in Bioengineering at the University of Maryland, College Park, joined the lab as part of the Nathan Schnaper Intern Program in Translational Cancer Research (NSIP).

Corinne is working on the genetic intersections between neoplasia and brain development, mining patient genome databases and using in vivo genome editing to probe effects on brain circuit development.

Congrats to Bek and Alex for Postdoctoral Fellow Excellence in Mentorship and Teacher of the Year Awards!

Bek Altas and Alex Poulopoulos were Awarded by the Graduate School of Life Sciences with the 2020 Postdoctoral Fellow Excellence in Mentorship Award and Teacher of the Year Award, a lab dynamic duo in mentorship!!!

Congrats to Ryan and co. for “Cas9 fusions for precision in vivo editing” out on the bioRxiv!

The lab’s first pub is a nifty piece of synth bio for genome editing the brain. Richardson et al. describe a platform to test and develop new high-precision genome editing reagents. Some of our new CRISPR fusions, like eRad18-Cas9-CtIP with linear donors, showed up to 45-times higher accuracy at point-mutation editing compared to vanilla CRISPR. Another step toward direct in vivo knockin and in situ gene therapy approaches!

https://www.biorxiv.org/content/10.1101/2020.07.15.199620v1

Look out for these PouLab super stars at UNC and UCSF !

And now some good news for a change… BIG CONGRATS to Marilyn Steyert and Uriel Jean-Baptist for cleaning house on their graduate school applications!

Come next semester, Marilyn will be a PhD student in the Biomedical Sciences (BMS) Program at UC San Francisco, and Uriel will be a PhD student in the Biological & Biomedical Sciences Program (BBSP) at UNC Chapel Hill. We will miss you both, and you both will make us proud!