Calling all Neuroscientists!!!

Come check out all the PouLab posters at SfN 2023

Sunday pm session: “Genetic Techniques to Target and/or Manipulate Cells”, three back-to-back posters :

• VV22: Find out what the best tag is for labeling your protein by Knockin at Andrea Romanowski‘s poster
  https://www.abstractsonline.com/pp8/#!/10892/presentation/39783
• VV23: Learn about how some genes are resistant to CRISPR-activation at Ashley Marquardt‘s poster
  https://www.abstractsonline.com/pp8/#!/10892/presentation/39785
• VV24: Get to see Neuroligin 3 for the first time in the human brain and how it might be used against brain cancer at Ro Whitten‘s poster
  https://www.abstractsonline.com/pp8/#!/10892/presentation/39784

Monday am session “Animal Models of Epilepsy: Genetics and Pharmacology”:

• C57: Learn about an exciting new way to model individual epilepsy patients in mice using prime editing from Colin Robertson‘s poster
  https://www.abstractsonline.com/pp8/#!/10892/presentation/32416

Wednesday pm session “Mechanisms Underlying Axon Growth and Targeting”:

• B14: Hear about a strange non-coding RNA that is involved in axon growth and potentially regeneration at Garrett Crutcher‘s poster
  https://www.abstractsonline.com/pp8/#!/10892/presentation/33556

Celebrate neuroscience with us!!!

Ryan Richardson’s paper describing the development of Cas9-RC, a new CRISPR agent with increased performance for knockin, is out in the October 2023 issue of The CRISPR Journal.

Not only that, but the paper got the cover! Kudos to Cheryl Brandenburg for the beautiful image of knockin neurons and astrocytes using Cas9-RC in the developing mouse brain. Congrats to the whole team for concluding this large piece of Synth Bio meets Dev Neurosci!

We would be delighted to have you try out Cas9-RC for your own knockin needs. Plasmids will become available on Addgene within the next few days!

Follow our science at the 4 PouLab posters at SfN 2022 in San Diego!

Cheryl Brandenburg on Monday morning (Y5):
“Light sheet mapping of parvalbumin subtypes of Purkinje cells using in vivo CRISPR strategies“

Colin Robertson on Monday afternoon (YY36):
“Neural somatic genome editing in the brain for personalized patient models“

Garrett Bunce and Ro Whitten on Wednesday morning (B31):
“The novel gene Ganon-1 produces a scaffolding RNA for growth signaling in developing axon projections“

Andrea Romanowski on Wednesday morning (C45):
“Mosaic Cas9 fusions to investigate cortical wiring by IgLON schizophrenia risk-genes in the rodent brain”

See you all at #SFN2022

Our work on how the synaptic adhesion molecule Neuroligin-3 is targeted to either excitatory or inhibitory synapses based on phosphorylation is now available on the bioRxiv! Congrats to Bekir Altas, Liam Tuffy, Annarita Patrizi and the rest of the team in this international collaboration between the University of Maryland School of Medicine, the Max Planck Institute for Multidisciplinary Sciences, and the University of Turin.

Phosphorylation Determines Whether Neuroligin-3 is at Excitatory or Inhibitory Synapses in Different Regions of the Brain

Garrett hosts NOVA’s Halal on the Lawn, then gives a talk on the lncRNA he discovered; Andrea does supply runs, then gives a talk on multiplexing schizophrenia risk genes with CRISPR; Elise handles donation logistics, and attends both talks! And at the end of a good day’s work, the lab celebrates Alex’s birthday.