Growth cone sorting in Nature Protocols

Our protocol on subtype-specific growth cone sorting by Engmann and Hatch et al. is out on Nature Protocols today from Alex’s old team in the Macklis lab@Harvard!

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.

Growth cone sorting from the cerebral cortex
Growing neuron projections forming circuitry linking the two brain hemispheres, labeled by in utero electroporation.