Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.
PouLab postdoc Cheryl Brandenburg was awarded a 2021 Autism Research Institute (ARI) grant for her work on “Cerebellar Circuits in 3D: Screening autism-associated genes in cleared brains with in utero CRISPR genome editing“. Congratulations Cheryl!!! Here is just a snippet of the mind-bending 3D cerebellar circuits from Cheryl’s in vivo edited Purkinje cells…!
Lab alum, Ryan R. Richardson, now a AAAS STP fellow at the NIH BRAIN Initiative, together with colleagues deputy Director Andrea C. Beckel-Mitchener, Director John Ngai, and program Director Devon C. Crawford, published a paper today in Neuron, outlining how BRAIN’s mission for scientific excellence is empowered by tapping into the full spectrum of diverse talents and perspectives. We’re grateful for your work in advancing opportunities and excelling innovative neuroscience research through inclusion and diversity!
Corinne Martin, a rising sophomore majoring in Bioengineering at the University of Maryland, College Park, joined the lab as part of the Nathan Schnaper Intern Program in Translational Cancer Research (NSIP).
Corinne is working on the genetic intersections between neoplasia and brain development, mining patient genome databases and using in vivo genome editing to probe effects on brain circuit development.
Bek Altas and Alex Poulopoulos were Awarded by the Graduate School of Life Sciences with the 2020 Postdoctoral Fellow Excellence in Mentorship Award and Teacher of the Year Award, a lab dynamic duo in mentorship!!!
Congratulations to PIN postdoctoral fellow Dr. Bekir Altas for winning the Postdoctoral Fellow Excellence in Mentorship Award for an outstanding record of supportive mentorship to PIN and GPILS trainees! pic.twitter.com/RJNx37O9lJ
— Univ of Maryland School of Medicine Neuroscience (@UMMedNeuro) October 26, 2020
Ryan Richardson, the lab’s first postdoc, has joined the NIH Office of the Director, BRAIN Initiative with a prestigious AAAS Science and Technology Policy Fellowship! Congratulations Ryan, we’re proud of you!!!
Read the press release of Ryan’s award:
The lab’s first pub is a nifty piece of synth bio for genome editing the brain. Richardson et al. describe a platform to test and develop new high-precision genome editing reagents. Some of our new CRISPR fusions, like eRad18-Cas9-CtIP with linear donors, showed up to 45-times higher accuracy at point-mutation editing compared to vanilla CRISPR. Another step toward direct in vivo knockin and in situ gene therapy approaches!