Neuroligin-3 paper out in Biological Psychiatry!

Just out, our paper on the biology of a synaptic adhesion molecule critically implicated in autism, published in Biological Psychiatry!

This massive paper includes outstanding work from scientists and collaborators at the Max Planck Institute for Multidisciplinary Sciences in Germany, and the University of Turin in Italy. It includes some of the first ever imaging of human synapses (spot the cool cup-shaped presynaptic terminals nestled in the human brainstem ????!), and identifies a molecular mechanism that determines the synaptic localization and transmitter-specificity of Neuroligin-3 between excitatory and inhibitory synapses in the brain. Congrats to everyone involved, a great way to end the year!

Region-Specific Phosphorylation Determines Neuroligin-3 Localization to Excitatory versus Inhibitory Synapses

 

Cas9-RC on the cover of The CRISPR Journal!

Ryan Richardson’s paper describing the development of Cas9-RC, a new CRISPR agent with increased performance for knockin, is out in the October 2023 issue of The CRISPR Journal.

Not only that, but the paper got the cover! Kudos to Cheryl Brandenburg for the beautiful image of knockin neurons and astrocytes using Cas9-RC in the developing mouse brain. Congrats to the whole team for concluding this large piece of Synth Bio meets Dev Neurosci!

We would be delighted to have you try out Cas9-RC for your own knockin needs. Plasmids will become available on Addgene within the next few days!

Cas9-RC Knockin on the cover of The CRISPR Journal

PouLab at SfN 2022

Follow our science at the 4 PouLab posters at SfN 2022 in San Diego!

Cheryl Brandenburg on Monday morning (Y5):
Light sheet mapping of parvalbumin subtypes of Purkinje cells using in vivo CRISPR strategies

Colin Robertson on Monday afternoon (YY36):
Neural somatic genome editing in the brain for personalized patient models

Garrett Bunce and Ro Whitten on Wednesday morning (B31):
The novel gene Ganon-1 produces a scaffolding RNA for growth signaling in developing axon projections

Andrea Romanowski on Wednesday morning (C45):
Mosaic Cas9 fusions to investigate cortical wiring by IgLON schizophrenia risk-genes in the rodent brain”


See you all at #SFN2022