Our paper with Gene Blatt in Journal of Comparative Neurology on the evolution of neuroanatomical tracing methods came out today with Cheryl’s beautiful electroporated, cleared, and light sheet imaged hippocampus gracing the cover, alongside Dee Pandya’s India Ink traces. Happy New Year everyone!
Just out, our paper on the biology of a synaptic adhesion molecule critically implicated in autism, published in Biological Psychiatry!
This massive paper includes outstanding work from scientists and collaborators at the Max Planck Institute for Multidisciplinary Sciences in Germany, and the University of Turin in Italy. It includes some of the first ever imaging of human synapses (spot the cool cup-shaped presynaptic terminals nestled in the human brainstem ????!), and identifies a molecular mechanism that determines the synaptic localization and transmitter-specificity of Neuroligin-3 between excitatory and inhibitory synapses in the brain. Congrats to everyone involved, a great way to end the year!
Region-Specific Phosphorylation Determines Neuroligin-3 Localization to Excitatory versus Inhibitory Synapses
Not only that, but the paper got the cover! Kudos to Cheryl Brandenburg for the beautiful image of knockin neurons and astrocytes using Cas9-RC in the developing mouse brain. Congrats to the whole team for concluding this large piece of Synth Bio meets Dev Neurosci!
We would be delighted to have you try out Cas9-RC for your own knockin needs. Plasmids will become available on Addgene within the next few days!
Follow our science at the 4 PouLab posters at SfN 2022 in San Diego!
Colin Robertson on Monday afternoon (YY36):
“Neural somatic genome editing in the brain for personalized patient models“
Garrett Bunce and Ro Whitten on Wednesday morning (B31):
“The novel gene Ganon-1 produces a scaffolding RNA for growth signaling in developing axon projections“
See you all at #SFN2022
PouLab postdoc Cheryl Brandenburg was awarded a 2021 Autism Research Institute (ARI) grant for her work on “Cerebellar Circuits in 3D: Screening autism-associated genes in cleared brains with in utero CRISPR genome editing“. Congratulations Cheryl!!! Here is just a snippet of the mind-bending 3D cerebellar circuits from Cheryl’s in vivo edited Purkinje cells…!