Garrett hosts NOVA’s Halal on the Lawn, then gives a talk on the lncRNA he discovered; Andrea does supply runs, then gives a talk on multiplexing schizophrenia risk genes with CRISPR; Elise handles donation logistics, and attends both talks! And at the end of a good day’s work, the lab celebrates Alex’s birthday.
Read all about our postulate of the curious little things called “mTOR outposts” in this Hypothesis & Theory paper just out in Frontiers in Cellular Neuroscience!
Happy day for Bek, Andrea, Garrett, and Alex. Appreciations to Helen Bateup and Akira Yoshii for very constructive reviewing; to Gerardo Morfini for editing the Kinase/phosphatase signaling and axonal function in health and disease topic; and to all in the acknowledgements for the fascinating discussions around the concepts in the mTOR outpost model. Be part of those discussions, send us your thoughts!
Two-out-of-two for the lab’s posters at this year’s Program in Neuroscience retreat! Colin Robertson got the “Most innovative experimental design” prize for his poster on “In Vivo Prime Editor Introduces Patient Epilepsy Mutation in the Rodent Brain to Recapitulate Seizures” and Andrea Romanowski accepted a crown and scepter for the “Rule a kingdom” prize for her poster on “Multiplexed manipulation of gene dosage of schizophrenia risk genes using Cas9 fusions changes layer position of cortical neurons”. Congrats PouLab grad students!!!
Congratulations to Philip Iffland and the Peter Crino Lab –with help from PouLab grad student Andrea Romanowski among the collaborator team– for the publication of a massive piece of work just out in Brain, spanning the fields of human genetics, cell biology, genome editing, electrophysiology, and brain development to identify the gene (NPRL3) and mechanisms that cause epilepsy in affected patients.
The lab’s first pub is a nifty piece of synth bio for genome editing the brain. Richardson et al. describe a platform to test and develop new high-precision genome editing reagents. Some of our new CRISPR fusions, like eRad18-Cas9-CtIP with linear donors, showed up to 45-times higher accuracy at point-mutation editing compared to vanilla CRISPR. Another step toward direct in vivo knockin and in situ gene therapy approaches!
(photo by T. Soykan)
The lab has fun recreating Raphael’s “The School of Athens” in Chicago, while presenting at the 2019 meeting of the Society for Neuroscience!
Ryan, Marilyn, and Jeffrey unveiled their new approach to high efficiency in vivo genome engineering with in situ CRISPR:
Bek, Andrea, Noury, and Uriel debuted their findings on how a mutation in a family with intellectual disability affects the dynactin complex and cortical circuit wiring:
Alex presented new findings on how phosphorylation of Neuroligin adhesion molecules determines synapse specificities:
And how reciprocal adhesion gradient matching guides the development of cortical circuitry:
… and Garrett stashed us some sweet top-shelf probes…
Andrea and Garrett spreading the love for neuroscience at Windsor Hills Elementary and Middle School!
Learn more at UMB NOVA (Neuroscience Outreach and Volunteer Association)
Do we use the windows as white boards? We sure do.
Today is the first day for Ben Grosso (Program in Biochemistry & Molecular Biology) and Garrett Bunce (Program in Neuroscience) in the lab, kicking off the first round of 2019 lab rotations for GPILS students. Andrea and Marilyn introduce the new lab members to the lab’s science…
Andrea Romanowski, from the Program in Molecular Medicine, becomes the lab’s 1st PhD student! Andrea’s first official day of PhD work, July 26, is also the day that, exactly 100 years ago, Emmy Noether published a paper outlining her eponymous theorem. Auspicious beginnings for the future Dr. Romanowski!